Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

Name: Anti-Honeybee Api m 4/Melittin Polyclonal Antibody
Brand: abinScience
SKU: ZA251014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

ZA251014

Description

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Apis mellifera (Honeybee)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).

Target

Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT

Purification

Purified by antigen affinity column.

Accession

P01501

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991

2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243

3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482

4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226

5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280

6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135

7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776

8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667

9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434

Note

For research use only.

Images

References

Datasheet

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us