Anti-EV71 VP4/P1A Polyclonal Antibody (VK633034)

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Datasheet

Overview

Catalog No.

VK633034

Description

Anti-EV71 VP4/P1A Polyclonal Antibody (VK633034) is a rabbit polyclonal antibody detecting EV71 VP4/P1A in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human enterovirus 71 (strain 7423/MS/87) (EV71) (EV-71)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human enterovirus 71 (EV71) VP4/P1A (Gly2-Lys69).

Target

Genome polyprotein, Protease 2A, P2A, 3.4.22.29, Picornain 2A, Protein 2A

Purification

Purified by antigen affinity column.

Accession

Q66479

RRID

Anti-EV71 VP4/P1A Polyclonal Antibody (abinScience Cat# VK633034, RRID:AB_3725103)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (VP4/P1A) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.

1. Zhou, D. et al. (2019) Nature microbiology 4, 414-419. PMID: 30531980

Note

For research use only.

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References

Datasheet