Anti-EV71 P3C/Protease 3C Polyclonal Antibody (VK613024)

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Overview
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Datasheet

Overview

Catalog No.

VK613024

Description

Anti-EV71 P3C/Protease 3C Polyclonal Antibody (VK613024) is a rabbit polyclonal antibody detecting 3C protease in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Enterovirus A71 (EV-A71)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human enterovirus 71 (EV71) P3C/Protease 3C (Gly1-Gln183(Val15Ile, Ile158Val)).

Target

3C protease, partial, 3C protease, partial

Purification

Purified by antigen affinity column.

Accession

B9VUU3

RRID

Anti-EV71 P3C/Protease 3C Polyclonal Antibody (abinScience Cat# VK613024, RRID:AB_3725104)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Genome polyprotein (P3C/Protease 3C) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.

Note

For research use only.

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References

Datasheet