Anti-CHPV P/Phosphoprotein Polyclonal Antibody (VK546014)

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50ug
100ug

Number:

Overview
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Datasheet

Overview

Catalog No.

VK546014

Description

Anti-CHPV P/Phosphoprotein Polyclonal Antibody (VK546014) is a rabbit polyclonal antibody detecting Phosphoprotein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Chandipura virus (strain I653514) (CHPV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Chandipura virus (CHPV) P/Phosphoprotein (Thr104-Asn293).

Target

Phosphoprotein, Protein P, Protein M1, P

Purification

Purified by antigen affinity column.

Accession

P16380

RRID

Anti-CHPV P/Phosphoprotein Polyclonal Antibody (abinScience Cat# VK546014, RRID:AB_3725148)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 4°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phosphoprotein is a ~32 kDa protein. Nonenzymatic cofactor regulating the function and conformation of the RNA polymerase and part of the transcription and replication complex. Binds the viral ribonucleocapsid and positions the L polymerase on the template. Acts as a chaperone for newly synthesized free N protein, so-called N(0). Plays a role in virion assembly.

1. Majumdar, A. et al. (2004) Biochemistry 43, 2863-70. PMID: 15005621

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-P-CHPV polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant CHPV P/Phosphoprotein (Catalog No: VK546012)

References

Datasheet

Anti-CHPV P/Phosphoprotein Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CHPV P/Phosphoprotein Polyclonal Antibody (VK546014)

Overview

Images

References

Datasheet

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