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Anti-Mycoplasma pneumoniae P30 adhesin Polyclonal Antibody (JN837014)

Anti-Mycoplasma pneumoniae P30 adhesin Polyclonal Antibody
Anti-Mycoplasma pneumoniae P30 adhesin Polyclonal Antibody
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Overview
Catalog No.JN837014
Description
Anti-Mycoplasma pneumoniae P30 adhesin Polyclonal Antibody (JN837014) is a rabbit polyclonal antibody detecting P30 adhesin in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityMycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Mycoplasma pneumoniae P30 adhesin (Lys101-Arg274).
Target P30 adhesin, 30 kDa adhesin-related protein, Cytadhesin P30, p30, MPN_453, MP388, Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)
Purification Purified by antigen affinity column.
Accession P75330
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

30 kDa adhesin-related protein (P30 adhesin) is a ~29 kDa protein. Adhesin necessary for successful cytadherence and virulence (Probable). The attachment organelle is involved in gliding cell motility and adhesion to host cells.

1. Layh-Schmitt, G. et al. (1997) FEMS microbiology letters 152, 101-8. PMID: 9228776
Note For research use only.
Images
  • Anti-Mycoplasma pneumoniae P30 adhesin Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-p30 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Mycoplasma pneumoniae P30 adhesin (Catalog No: JN837012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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