Anti-Human TSHR Antibody (SAA0401) (HB852013)

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1mg

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Overview
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References
Datasheet

Overview

Catalog No.

HB852013

Description

Anti-Human TSHR Antibody (SAA0401) [SAA0401] (HB852013) is a mouse monoclonal antibody detecting TSHR in ELISA, FCM, IHC, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, IHC, WB

Host species

Mouse

Isotype

IgG2a, lambda

Clone ID

SAA0401

Clonality

Monoclonal

Target

Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P16473

RRID

Anti-Human TSHR Antibody (SAA0401) (abinScience Cat# HB852013, RRID:AB_3725206)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS).

1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099

2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258

3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205

4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204

5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # HB852013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human TSHR Antibody (SAA0401).
Western blot

Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse ovary lysate