Anti-Mouse MMP3 Recombinant Antibody (CH01) (MY526013)

Name: Anti-Mouse MMP3 Recombinant Antibody (CH01)
Brand: abinScience
SKU: MY526013
Price: 110 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

MY526013

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IF

Host species

Rat

Isotype

IgG1, kappa

Clone ID

CH01

Clonality

Monoclonal

Target

SL-1, Transin-1, MMP-3, MMP3, Matrix metalloproteinase-3, Stromelysin-1, STMY1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P28862

RRID

Anti-Mouse MMP3 Antibody (CH01) (abinScience Cat# MY526013, RRID:AB_3725632)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Stromelysin-1 (MMP3) is a ~53 kDa protein. Metalloproteinase with a rather broad substrate specificity that can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates different molecules including growth factors, plasminogen or other matrix metalloproteinases such as MMP9. Once released into the extracellular matrix (ECM), the inactive pro-enzyme is activated by the plasmin cascade signaling pathway. Also acts intracellularly. For example, in dopaminergic neurons, gets activated by the serine protease HTRA2 upon stress and plays a pivotal role in DA neuronal degeneration by mediating microglial activation and alpha-synuclein/SNCA cleavage.

1. Kim, YS. et al. (2007) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21, 179-87. PMID: 17116747

2. Feng, T. et al. (2022) Antiviral research 206, 105388. PMID: 35940311

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse MMP3 Antibody (CH01)
Bioactivity

Detects recombinant human MMP3 (Catalog No: HY526012) in indirect ELISA
Flow cytometry

Flow-cytometry using anti-human MMP3 antibody. HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP3 monoclonal antibody (Catalog MY526013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: RF690334) and cells analysed on a NovoCyte Flow Cytometer.