Anti-Mouse GPC1 Antibody (SAA0273) (MW614013)

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Datasheet

Overview

Catalog No.

MW614013

Description

Anti-Mouse GPC1 Antibody (SAA0273) [SAA0273] (MW614013) is a mouse monoclonal antibody detecting GPC1 in ELISA, FCM. Suitable for Human and Mouse.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human, Mouse

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

SAA0273

Clonality

Monoclonal

Target

Glypican-1, GPC1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9QZF2

RRID

Anti-Mouse GPC1 Antibody (SAA0273) (abinScience Cat# MW614013, RRID:AB_3725637)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Glypican-1 (GPC1) is a ~61 kDa protein. Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination. May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. Binds Cu(2+) or Zn(2+) ions.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse GPC1 Antibody (SAA0273).

References

Datasheet

Anti-Mouse GPC1 Antibody (SAA0273).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse GPC1 Antibody (SAA0273) (MW614013)

Overview

Images

References

Datasheet

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