Anti-Human APOL1/Apolipoprotein L1, L2 Antibody (3.6D12) (HT291013)

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Datasheet

Overview

Catalog No.

HT291013

Description

Anti-Human APOL1/Apolipoprotein L1, L2 Antibody (3.6D12) [3.6D12] (HT291013) is a mouse monoclonal antibody detecting Apolipoprotein L1 in Blocking, ELISA. Suitable for Human.

Species reactivity

Human

Applications

Blocking, ELISA

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

3.6D12

Clonality

Monoclonal

Target

Apolipoprotein L1, ApoL-I, ApoL, APOL1, APOL, Apolipoprotein L-I, Apo-L, Apolipoprotein L, ApoL-II, Apolipoprotein L-II, APOL2, Apolipoprotein L2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O14791, Q9BQE5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Apolipoprotein L1 (APOL1) is a ~43 kDa protein. May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. A component of trypanosome lytic factor of human serum; plays a crucial role in killing Trypanosoma brucei by forming pores in parasite lysosomal membranes and sensitizing T.brucei to oxidation-stimulated osmotic lysis.

1. Vanhamme, L. et al. (2003) Nature 422, 83-7. PMID: 12621437

2. Pérez-Morga, D. et al. (2005) Science (New York, N.Y.) 309, 469-72. PMID: 16020735

3. Lecordier, L. et al. (2009) PLoS pathogens 5, e1000685. PMID: 19997494

4. Greene, AS. et al. (2016) The Journal of biological chemistry 291, 3063-75. PMID: 26645690

5. Tytler, EM. et al. (1995) Molecular and biochemical parasitology 69, 9-17. PMID: 7723792

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human APOL1/Apolipoprotein?L1, L2 Antibody (3.6D12).

References

Datasheet

Anti-Human APOL1/Apolipoprotein L1, L2 Antibody (3.6D12).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human APOL1/Apolipoprotein L1, L2 Antibody (3.6D12) (HT291013)

Overview

Images

References

Datasheet

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