Anti-Human CCL20/MIP-3-alpha Antibody (SAA0277) (HX131013)

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Overview
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Datasheet

Overview

Catalog No.

HX131013

Description

Anti-Human CCL20/MIP-3-alpha Antibody (SAA0277) [SAA0277] (HX131013) is a mouse monoclonal antibody detecting MIP-3-alpha in Blocking, ELISA, FCM. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

Blocking, ELISA, FCM

Host species

Mouse

Isotype

IgG1, kappa

Clone ID

SAA0277

Clonality

Monoclonal

Target

Beta-chemokine exodus-1, SCYA20, LARC, C-C motif chemokine 20, Liver and activation-regulated chemokine, Small-inducible cytokine A20, MIP-3-alpha, CCL20, MIP3A, Macrophage inflammatory protein 3 alpha, CC chemokine LARC

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P78556

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

C-C motif chemokine 20 (CCL20/MIP-3-alpha) is a ~10 kDa protein. Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation.

1. Schutyser, E. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 165, 4470-7. PMID: 11035086

2. Nelson, RT. et al. (2001) Genomics 73, 28-37. PMID: 11352563

3. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036

4. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174

5. Hieshima, K. et al. (1997) The Journal of biological chemistry 272, 5846-53. PMID: 9038201

6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988

7. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636

8. Hromas, R. et al. (1997) Blood 89, 3315-22. PMID: 9129037

Note

For research use only.

Images

Flow Cytometry

Flow-cytometry using anti-human CCL20 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL20 antibody monoclonal antibody (Catalog # HX131013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CCL20/MIP-3-alpha Antibody (SAA0277).