Anti-Phospho-AKT1 (pS473) Antibody (P8.H9) (MB610013)

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Datasheet

Overview

Catalog No.

MB610013

Description

Anti-Phospho-AKT1 (pS473) Antibody (P8.H9) [P8.H9] (MB610013) is a rat monoclonal antibody detecting AKT1 in ELISA, IHC, IP, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, AKT8 murine leukemia virus

Applications

ELISA, IHC, IP, WB

Host species

Rat

Isotype

IgG1, kappa

Clone ID

P8.H9

Clonality

Monoclonal

Target

RAC-alpha serine/threonine-protein kinase, RAC, RAC-PK-alpha, PKB alpha, Proto-oncogene c-Akt, Protein kinase B alpha, Protein kinase B, AKT1, PKB

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P31749

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

RAC-alpha serine/threonine-protein kinase (p-AKT1) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.

1. Nicholson, KM. et al. (2002) Cellular signalling 14, 381-95. PMID: 11882383

2. Rönnstrand, L. (2004) Cellular and molecular life sciences : CMLS 61, 2535-48. PMID: 15526160

3. Cartlidge, RA. et al. (2005) The EMBO journal 24, 1696-705. PMID: 15861136

4. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781

5. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960

6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173

7. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641

Note

For research use only.

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