Anti-Phospho-Smad2 (pT8) Recombinant Antibody (P4.B9) (HS710013)

Name: Anti-Phospho-Smad2 (pT8) Recombinant Antibody (P4.B9)
Brand: abinScience
SKU: HS710013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HS710013

Species reactivity

Human, Mouse, Rat, Bovine, Sumatran orangutan, Zebrafish

Applications

IHC, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

P4.B9

Clonality

Monoclonal

Target

Mothers against decapentaplegic homolog 2, SMAD 2, SMAD2, JV18-1, Mad-related protein 2, hMAD-2, MADR2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, Smad2, hSMAD2, MAD homolog 2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q15796

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

SMAD family member 2 (p-Smad2) is a ~52 kDa protein. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma.

1. Eppert, K. et al. (1996) Cell 86, 543-52. PMID: 8752209

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phospho-Smad2 (pT8) Antibody (P4.B9).

References

Datasheet

Anti-Phospho-Smad2 (pT8) Recombinant Antibody (P4.B9).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phospho-Smad2 (pT8) Recombinant Antibody (P4.B9) (HS710013)

Overview

Images

References

Datasheet

SDS

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