Anti-Phospho-Smad3 (pS208/pS213) Antibody (5A11) (HX888023)

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50ug
100ug
1mg

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Datasheet

Overview

Catalog No.

HX888023

Description

Anti-Phospho-Smad3 (pS208/pS213) Antibody (5A11) [5A11] (HX888023) is a mouse monoclonal antibody detecting Smad3 in IHC, IP, WB. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

IHC, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

5A11

Clonality

Monoclonal

Target

Mad3, JV15-2, Mothers against decapentaplegic homolog 3, SMAD family member 3, Smad3, SMAD3, MADH3, hMAD-3, SMAD 3, MAD homolog 3, hSMAD3, Mothers against DPP homolog 3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P84022

RRID

Anti-Phospho-Smad3 (pS208/pS213) Antibody (5A11) (abinScience Cat# HX888023, RRID:AB_3725727)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

SMAD family member 3 (p-Smad3) is a ~48 kDa protein. Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive.

Note

For research use only.

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