Anti-Phospho-HTRA2 (pS212) Recombinant Antibody (P6.C12) (HT447013)

Name: Anti-Phospho-HTRA2 (pS212) Recombinant Antibody (P6.C12)
Brand: abinScience
SKU: HT447013
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT447013

Species reactivity

Human

Applications

IHC, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

P6.C12

Clonality

Monoclonal

Target

High temperature requirement protein A2, OMI, HtrA2, PRSS25, Serine protease 25, Serine proteinase OMI, Serine protease HTRA2, mitochondrial, HTRA2, Omi stress-regulated endoprotease

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O43464

RRID

Anti-Phospho-HTRA2 (pS212) Antibody (P6.C12) (abinScience Cat# HT447013, RRID:AB_3725755)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Serine protease HTRA2, mitochondrial (pHTRA2) is a ~48 kDa protein. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6. Cleaves THAP5 and promotes its degradation during apoptosis.

1. Savopoulos, JW. et al. (2000) Protein expression and purification 19, 227-34. PMID: 10873535

2. Bartke, T. et al. (2004) Molecular cell 14, 801-11. PMID: 15200957

3. Hunkeler, M. et al. (2023) Science (New York, N.Y.) 379, 1105-1111. PMID: 36758104

4. Ehrmann, JF. et al. (2023) Science (New York, N.Y.) 379, 1117-1123. PMID: 36758105

5. Balakrishnan, MP. et al. (2009) American journal of physiology. Heart and circulatory physiology 297, H643-53. PMID: 19502560

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phospho-HTRA2 (pS212) Antibody (P6.C12)

References

Datasheet

Anti-Phospho-HTRA2 (pS212) Recombinant Antibody (P6.C12).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phospho-HTRA2 (pS212) Recombinant Antibody (P6.C12) (HT447013)

Overview

Images

References

Datasheet

SDS

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