Anti-Phospho-CHK2 (pT68) Recombinant Antibody (34#) (HF505013)

Name: Anti-Phospho-CHK2 (pT68) Recombinant Antibody (34#)
Brand: abinScience
SKU: HF505013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF505013

Species reactivity

Human

Applications

IHC, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

34#

Clonality

Monoclonal

Target

RAD53, CHK2 checkpoint homolog, hCds1, Serine/threonine-protein kinase Chk2, CDS1, Checkpoint kinase 2, CHEK2, Hucds1, Cds1 homolog, CHK2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O96017

RRID

Anti-Phospho-CHK2 (pT68) Antibody (34#) (abinScience Cat# HF505013, RRID:AB_3725761)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Serine/threonine-protein kinase Chk2 (p-CHK2) is a ~60 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression.

1. Liu, J. et al. (2023) Cell reports 42, 113402. PMID: 37943659

2. Magni, M. et al. (2014) Nucleic acids research 42, 13150-60. PMID: 25361978

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phospho-CHK2 (pT68) Antibody (34#).

References

Datasheet

Anti-Phospho-CHK2 (pT68) Recombinant Antibody (34#).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phospho-CHK2 (pT68) Recombinant Antibody (34#) (HF505013)

Overview

Images

References

Datasheet

SDS

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