Anti-Phospho-STAT3 (pY705) Antibody (SAA0302) (HW708013)

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1mg

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Datasheet

Overview

Catalog No.

HW708013

Description

Anti-Phospho-STAT3 (pY705) Antibody (SAA0302) [SAA0302] (HW708013) is a mouse monoclonal antibody detecting STAT3 in ELISA, IP, WB. Suitable for Human and Mouse.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IP, WB

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA0302

Clonality

Monoclonal

Target

Signal transducer and activator of transcription 3, APRF, STAT3, Acute-phase response factor

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P40763

RRID

Anti-Phospho-STAT3 (pY705) Antibody (SAA0302) (abinScience Cat# HW708013, RRID:AB_3725763)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Signal transducer and activator of transcription 3 (p-STAT3) is a ~88 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Upon activation of IL6ST/gp130 signaling by interleukin-6 (IL6), binds to the IL6-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

1. Tsai, YT. et al. (2000) Molecular and cellular biology 20, 2043-54. PMID: 10688651

2. Yamamoto, T. et al. (2002) Biochemical and biophysical research communications 297, 811-7. PMID: 12359225

3. Shao, H. et al. (2003) Cancer research 63, 3923-30. PMID: 12873986

4. Dreuw, A. et al. (2004) The Journal of biological chemistry 279, 36112-20. PMID: 15194700

5. Yuan, ZL. et al. (2005) Science (New York, N.Y.) 307, 269-73. PMID: 15653507

6. Ray, S. et al. (2005) Gastroenterology 129, 1616-32. PMID: 16285960

7. Saxena, NK. et al. (2007) The Journal of biological chemistry 282, 13316-25. PMID: 17344214

8. Jiang, H. et al. (2008) Biochemical and biophysical research communications 368, 1-5. PMID: 18242580

9. Hou, T. et al. (2008) The Journal of biological chemistry 283, 30725-34. PMID: 18782771

10. Gao, X. et al. (2012) Molecular cell 45, 598-609. PMID: 22306293

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Phospho-STAT3 (pY705) Antibody (SAA0302)

References

Datasheet

Anti-Phospho-STAT3 (pY705) Antibody (SAA0302).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Phospho-STAT3 (pY705) Antibody (SAA0302) (HW708013)

Overview

Images

References

Datasheet

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