Anti-EBV/HHV-4 EBNA1/BKRF1 Recombinant Antibody (3D4) (VK686013)

Name: Anti-EBV/HHV-4 EBNA1/BKRF1 Recombinant Antibody (3D4)
Brand: abinScience
SKU: VK686013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK686013

Description

Anti-EBV/HHV-4 EBNA1/BKRF1 Recombinant Antibody (3D4) (VK686013) is a mouse monoclonal antibody detecting EBNA1/BKRF1 in ELISA, IF, WB. Suitable for Epstein-Barr virus (HHV-4) (Human herpesvirus 4).

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

Applications

ELISA, IF, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

3D4

Target

Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03211

RRID

Anti-EBV/HHV-4 EBNA1/BKRF1 Antibody (3D4) (abinScience Cat# VK686013, RRID:AB_3725809)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.

1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791

2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781

3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969

4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669

5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585

6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058

7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-EBV/HHV-4 EBNA1/BKRF1 Antibody (3D4)

References

Datasheet

Anti-EBV/HHV-4 EBNA1/BKRF1 Recombinant Antibody (3D4).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EBV/HHV-4 EBNA1/BKRF1 Recombinant Antibody (3D4) (VK686013)

Overview

Images

References

Datasheet

SDS