Anti-Human CTSL Recombinant Antibody (3D8) (HY099013)

Name: Anti-Human CTSL Recombinant Antibody (3D8)
Brand: abinScience
SKU: HY099013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY099013

Description

Anti-Human CTSL Recombinant Antibody (3D8) (HY099013) is a mouse monoclonal antibody detecting CTSL in ELISA, FCM, IF, IP, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, FCM, IF, IP, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

3D8

Tested applications

WB, FCM

Target

CTSL1, MEP, Procathepsin L, Major excreted protein, Cathepsin L1, CTSL

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P07711

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Procathepsin L (CTSL) is a ~37 kDa protein. Thiol protease important for the overall degradation of proteins in lysosomes (Probable). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells.

1. Felbor, U. et al. (2000) The EMBO journal 19, 1187-94. PMID: 10716919

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

Bioactivity

Detects CTSL in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CTSL antibody.CTSL Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CTSL antibody monoclonal antibody (Catalog # HY099013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CTSL Antibody (3D8).
Western blot

Western blot analysis was performed using anti-CTSL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CTSL (Catalog No: HY099011)
Lane 2: negative control

References

Datasheet

Anti-Human CTSL Recombinant Antibody (3D8).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CTSL Recombinant Antibody (3D8) (HY099013)

Overview

Images

References

Datasheet

SDS