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Anti-Human AFP/Alpha-fetoprotein Antibody (AF5) (HY276013)

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  • 100ug
  • 1mg
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Overview
Catalog No.HY276013
Species reactivityHuman
ApplicationsFCM, ICC, IF, WB
Host speciesMouse
IsotypeIgG1, kappa
Clone IDAF5
Clonality Monoclonal
Target Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, AFP, HPAFP
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P02771
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images

  • Bioactivity

    SEC-HPLC detection for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).

  • Flow Cytometry

    Flow-cytometry using anti-human AFP antibody.AFP Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AFP antibody monoclonal antibody (Catalog # HY276013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human AFP/Alpha-fetoprotein Antibody (AF5).

  • Flow CytoMetry

    Flow-cytometry using PE anti-human AFP antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human AFP monoclonal antibody (Catalog: HY276013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Western blot

    Western blot analysis was performed using anti-AFP/Alpha-fetoprotein monoclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant human AFP/Alpha-fetoprotein (Catalog No: HY276011)
    Lane 2: negative control

References
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Anti-Human AFP/Alpha-fetoprotein Antibody (AF5)

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