Anti-Human ApoE Recombinant Antibody (SAA0799) (HF739013)

Name: Anti-Human ApoE Recombinant Antibody (SAA0799)
Brand: abinScience
SKU: HF739013
Price: 110 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF739013

Description

Anti-Human ApoE Recombinant Antibody (SAA0799) (HF739013) is a mouse monoclonal antibody detecting ApoE in ELISA, FuncS, IHC, IP. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, FuncS, IHC, IP

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone ID

SAA0799

Target

Apolipoprotein E, Apo-E, APOE

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02649

RRID

Anti-Human APOE Antibody (SAA0799) (abinScience Cat# HF739013, RRID:AB_3725861)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Apolipoprotein E (APOE) is a ~36 kDa protein. APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.

1. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908

2. Arnon, R. et al. (1991) Biochimica et biophysica acta 1085, 336-42. PMID: 1911868

3. Marcel, YL. et al. (1983) Biochimica et biophysica acta 750, 411-7. PMID: 6860692

4. Sehayek, E. et al. (1991) The Journal of biological chemistry 266, 18259-67. PMID: 1917954

5. Verghese, PB. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, E1807-16. PMID: 23620513

6. Kowal, RC. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 5810-4. PMID: 2762297

7. Ji, ZS. et al. (1997) The Journal of biological chemistry 272, 31285-92. PMID: 9395455

8. Li, X. et al. (2003) Biochemistry 42, 10406-17. PMID: 12950167

Note

For research use only.

Images

Bioactivity

Detects APOE4 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human APOE Antibody (SAA0799).

References

Datasheet

Anti-Human ApoE Recombinant Antibody (SAA0799).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human ApoE Recombinant Antibody (SAA0799) (HF739013)

Overview

Images

References

Datasheet

SDS