Anti-Influenza A virus Nucleoprotein Recombinant Antibody (SAA0411) (VK528023) is a human monoclonal antibody detecting Nucleoprotein in ELISA, IF, WB. Suitable for Influenza A virus (H5N1), Influenza A virus (H1N1), Influenza A virus (H3N2), Influenza A virus (H4N6), Influenza A virus (H10N7), Influenza A virus (H11N9), Influenza A virus (H12N5), Influenza A virus (H13N6), Influenza A virus (H9N2), Influenza A virus (H8N4), Influenza A virus (H6N2), Influenza A virus (H7N8), Influenza A virus (H5N3).
Highlights
●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.
Species reactivity
Influenza A virus (H5N1), Influenza A virus (H1N1), Influenza A virus (H3N2), Influenza A virus (H4N6), Influenza A virus (H10N7), Influenza A virus (H11N9), Influenza A virus (H12N5), Influenza A virus (H13N6), Influenza A virus (H9N2), Influenza A virus (H8N4), Influenza A virus (H6N2), Influenza A virus (H7N8), Influenza A virus (H5N3)
Applications
ELISA, IF, WB
Host species
Human
Clonality
Monoclonal
Isotype
IgG1
Clone ID
SAA0411
Target
Nucleoprotein, Nucleocapsid protein, Protein N, NP, Influenza Virus (Flu)
Endotoxin level
< 10 EU/mg
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application
Dilution
ELISA
1:2000-1:20000
WB
1:500-1:2000
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background
Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Anti-Influenza A virus NP/Nucleoprotein Antibody (SAA0411).
中文
English
한국어
日本語
Español
Français
Русский
