Anti-Human VEGFA Antibody (SAA0531) (HB941013)

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Overview

Catalog No.	HB941013
Species reactivity	Human
Applications	ELISA, FCM
Host species	Rabbit
Isotype	IgG
Clone ID	SAA0531
Clonality	Monoclonal
Target	Vascular endothelial growth factor A, VPF, VEGFA, VEGF, Vascular permeability factor, VEGF-A
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15692
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note	For research use only.

Images

Bioactivity

Detects Human VEGFA in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human VEGFA antibody.VEGFA Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human VEGFA antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human VEGFA antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human VEGFA antibody monoclonal antibody (Catalog # HB941013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-rabbit antibody (Catalog # TF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet