Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203) (HF876013)

Name: Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203)
Brand: abinScience
SKU: HF876013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HF876013

Description

Anti-Human SOD1 Nanobody (SAA1203) [SAA1203] (HF876013) is a alpaca monoclonal antibody detecting SOD1 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA, WB

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1203

Tested applications

WB, IF

Target

Superoxide dismutase [Cu-Zn], SOD1, hSod1, Superoxide dismutase 1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P00441

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Superoxide dismutase [Cu-Zn] (SOD1) is a ~15 kDa protein. Destroys radicals which are normally produced within the cells and which are toxic to biological systems. Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive sulfur species (RSS) such as persulfides and polysulfides.

1. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062

2. Switzer, CH. et al. (2023) Proceedings of the National Academy of Sciences of the United States of America 120, e2205044120. PMID: 36630448

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SOD1 Nanobody (SAA1203)
Bioactivity

Detects recombinant SOD1 (Catalog No: HF876012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-SOD1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human SOD1 (Catalog No: HF876012)
Immunofluorescence

Immunofluorescence staining of PC12 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cytoplasm .

References

Datasheet

Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SOD1/Cu-Zn SOD Recombinant Nanobody (SAA1203) (HF876013)

Overview

Images

References

Datasheet

SDS