Anti-Human CAT/Catalase Recombinant Nanobody (SAA1202) (HY348013)

Name: Anti-Human CAT/Catalase Recombinant Nanobody (SAA1202)
Brand: abinScience
SKU: HY348013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY348013

Description

Anti-Human CAT/Catalase Nanobody (SAA1202) [SAA1202] (HY348013) is a alpaca monoclonal antibody detecting Catalase in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1202

Tested applications

Target

Catalase, CAT

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P04040

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Catalase is a ~59 kDa protein. Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

1. Takeuchi, A. et al. (1995) Cancer research 55, 1586-9. PMID: 7882369

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CAT/Catalase Nanobody (SAA1202)
Bioactivity

Detects recombinant catalase (Catalog No: HY348012) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Peroxisome matrix.

References

Datasheet

Anti-Human CAT/Catalase Recombinant Nanobody (SAA1202).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CAT/Catalase Recombinant Nanobody (SAA1202) (HY348013)

Overview

Images

References

Datasheet

SDS