Anti-Human Cannabinoid R1/CB1/CNR1 Recombinant Nanobody (SAA1274) (HB822013)

Name: Anti-Human Cannabinoid R1/CB1/CNR1 Recombinant Nanobody (SAA1274)
Brand: abinScience
SKU: HB822013
Price: 126 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB822013

Description

Anti-Human CNR1/CB1 Nanobody (SAA1274) [SAA1274] (HB822013) is a alpaca monoclonal antibody detecting CNR1 in ELISA, RIA, SPR. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, RIA, SPR, WB

Host species

Alpaca

Clonality

Monoclonal

Isotype

VHH-8His-Cys-tag

Clone ID

SAA1274

Tested applications

WB, IF

Target

CNR1, CB1, CANN6, CB-R, CNR, Cannabinoid receptor 1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P21554

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cannabinoid receptor 1 (CNR1/CB1) is a ~52 kDa protein. G-protein coupled receptor for endogenous cannabinoids (eCBs), including N-arachidonoylethanolamide (also called anandamide or AEA) and 2-arachidonoylglycerol (2-AG), as well as phytocannabinoids, such as delta(9)-tetrahydrocannabinol (THC). Mediates many cannabinoid-induced effects, acting, among others, on food intake, memory loss, gastrointestinal motility, catalepsy, ambulatory activity, anxiety, chronic pain. Signaling typically involves reduction in cyclic AMP. In the hypothalamus, may have a dual effect on mitochondrial respiration depending upon the agonist dose and possibly upon the cell type. Increases respiration at low doses, while decreases respiration at high doses.

1. Ryberg, E. et al. (2005) FEBS letters 579, 259-64. PMID: 15620723

2. Hua, T. et al. (2016) Cell 167, 750-762.e14. PMID: 27768894

3. Shao, Z. et al. (2016) Nature 540, 602-606. PMID: 27851727

4. Yang, X. et al. (2022) Nature chemical biology 18, 831-840. PMID: 35637350

5. Gérard, CM. et al. (1991) The Biochemical journal 279 ( Pt 1), 129-34. PMID: 1718258

6. Oddi, S. et al. (2012) British journal of pharmacology 165, 2635-51. PMID: 21895628

7. Niehaus, JL. et al. (2007) Molecular pharmacology 72, 1557-66. PMID: 17895407

8. Jourdan, T. et al. (2013) Nature medicine 19, 1132-40. PMID: 23955712

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CNR1/CB1 Nanobody (SAA1274)
Bioactivity

Detects recombinant CNR1 (Catalog No: HB822012) in indirect ELISA.
Western blot

Western blot analysis was performed using anti-CNR1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CNR1 (Catalog No: HB822012)
Immunofluorescence

Immunofluorescence staining of SHSY5Y with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane，Cytoplasm.

References

Datasheet

Anti-Human Cannabinoid R1/CB1/CNR1 Recombinant Nanobody (SAA1274).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human Cannabinoid R1/CB1/CNR1 Recombinant Nanobody (SAA1274) (HB822013)

Overview

Images

References

Datasheet

SDS