Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997) (JN937023)

Name: Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997)
Brand: abinScience
SKU: JN937023
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN937023

Description

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Nanobody (SAA0997) [SAA0997] (JN937023) is a alpaca monoclonal antibody detecting Internalin B in ELISA.

Species reactivity

Listeria monocytogenes

Applications

ELISA

Host species

Alpaca

Isotype

VHH-8His-Cys-tag

Clone ID

SAA0997

Clonality

Monoclonal

Target

Internalin B, InlB, Invasion protein InlB, inlB

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Purified by Nickel column.

Accession

P0DQD3

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Internalin B (InlB) is a ~71 kDa protein. Mediates the entry of L.monocytogenes into normally non-phagocytic mammalian host cells (Probable). Its host receptor is hepatocyte growth factor receptor (HGF receptor, a tyrosine kinase, MET) which is tyrosine-phosphorylated in response to InlB in human, green monkey, mouse and dog cell lines. Downstream adapter proteins GAB1 and CBL are phosphorylated in response to InlB, which also causes cell colony scattering. InlB binding to mammalian cells is saturable and inhibited by EDTA; InlB-coated beads can be taken up by host cells. Complement component 1 Q subcomponent-binding protein (gC1q-R, C1QBP) might act as an InlB receptor, leading to activation of PI3-kinase in green monkey cells.

1. Shen, Y. et al. (2000) Cell 103, 501-10. PMID: 11081636

2. Braun, L. et al. (1997) Molecular microbiology 25, 285-94. PMID: 9282740

3. Banerjee, M. et al. (2004) Molecular microbiology 52, 257-71. PMID: 15049825

4. Braun, L. et al. (2000) The EMBO journal 19, 1458-66. PMID: 10747014

5. Marino, M. et al. (2002) The EMBO journal 21, 5623-34. PMID: 12411480

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Nanobody (SAA0997)

References

Datasheet

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Listeria monocytogenes serotype 1/2a InlB/Internalin B Recombinant Nanobody (SAA0997) (JN937023)

Overview

Images

References

Datasheet

SDS

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