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Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3) (HB102013)

Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3)
Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3)
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Overview
Catalog No.HB102013
Description
Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3) [SM3] (HB102013) is a mouse monoclonal antibody detecting CD227 in ELISA, FCM, IF, IHC-P, IP, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • Multi-Application — Validated across multiple applications.
  • Flow Cytometry — Validated for flow cytometry applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, FCM, IF, IHC-P, IP, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSM3
Clonality Monoclonal
Target PEMT, CA 15-3, H23AG, Peanut-reactive urinary mucin, PEM, EMA, Cancer antigen 15-3, MUC-1, Episialin, MUC1-CT, MUC1-beta, Tumor-associated mucin, CD227, Polymorphic epithelial mucin, KL-6, MUC1-alpha, MUC1, PUM, Krebs von den Lungen-6, MUC1-NT, Tumor-associated epithelial membrane antigen, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P15941
RRID

Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3) (abinScience Cat# HB102013, RRID:AB_3726898)

Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Note For research use only.
Images
  • Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3)

    SDS-PAGE

    SDS-PAGE for Anti-Human/Mouse/Rat CD227/MUC1 Antibody (SM3).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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