Anti-Human RHO/Rhodopsin Antibody (K42-41L) (HY021013)

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Spec:

50ug
100ug
1mg

Number:

Overview
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Datasheet

Overview

Catalog No.

HY021013

Description

Anti-Human RHO/Rhodopsin Antibody (K42-41L) [K42-41L] (HY021013) is a mouse monoclonal antibody detecting Rhodopsin in Blocking, ELISA, ICC. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

Blocking, ELISA, ICC

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

K42-41L

Clonality

Monoclonal

Target

Rhodopsin, OPN2, Opsin-2, RHO

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P08100

RRID

Anti-Human RHO/Rhodopsin Antibody (K42-41L) (abinScience Cat# HY021013, RRID:AB_3726901)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Rhodopsin is a ~38 kDa protein. Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

1. Sieving, PA. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 880-4. PMID: 7846071

2. Rao, VR. et al. (1994) Nature 367, 639-42. PMID: 8107847

3. Noorwez, SM. et al. (2003) The Journal of biological chemistry 278, 14442-14450. PMID: 12566452

4. Dryja, TP. et al. (1990) The New England journal of medicine 323, 1302-7. PMID: 2215617

5. Kang, Y. et al. (2015) Nature 523, 561-7. PMID: 26200343

6. He, Y. et al. (2017) Cell research 27, 728-747. PMID: 28524165

7. Zhou, XE. et al. (2017) Cell 170, 457-469.e13. PMID: 28753425

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human RHO/Rhodopsin Antibody (K42-41L)

References

Datasheet

Anti-Human RHO/Rhodopsin Antibody (K42-41L).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RHO/Rhodopsin Antibody (K42-41L) (HY021013)

Overview

Images

References

Datasheet

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