Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3) (VK492053)

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Datasheet

Overview

Catalog No.

VK492053

Description

Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3) [A3] (VK492053) is a human monoclonal antibody detecting Capsid protein p24 in ELISA, WB.

Species reactivity

Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (HIV-1)

Applications

ELISA, WB

Host species

Human

Isotype

IgG1

Clone ID

Clonality

Monoclonal

Target

Pr160Gag-Pol, Gag-Pol polyprotein, gag-pol, Capsid protein p24, CA

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03366

RRID

Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3) (abinScience Cat# VK492053, RRID:AB_3726934)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Gag-Pol polyprotein (Capsid protein p24/CA) is a ~163 kDa protein. Mediates, with Gag polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi). Gag-Pol polyprotein may regulate its own translation, by the binding genomic RNA in the 5'-UTR. At low concentration, the polyprotein would promote translation, whereas at high concentration, the polyprotein would encapsidate genomic RNA and then shut off translation.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3).

References

Datasheet

Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-HIV-1 Capsid protein p24/CA protein Antibody (A3) (VK492053)

Overview

Images

References

Datasheet

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