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Anti-Mouse Tau Recombinant Antibody (TAU-5) (MY086013)

Anti-Mouse Tau Recombinant Antibody (TAU-5)
Anti-Mouse Tau Recombinant Antibody (TAU-5)
Anti-Mouse Tau Recombinant Antibody (TAU-5)
Anti-Mouse Tau Recombinant Antibody (TAU-5)
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Overview
Catalog No.MY086013
Species reactivityBovine, Human
ApplicationsIF, IHC-P, IP, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDTAU-5
Tested applications FCM
Target MAPT, MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau, TAU
Endotoxin level < 10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P10637
RRID Anti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5) (abinScience Cat# MY086013, RRID:AB_3726999)
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Microtubule-associated protein tau (MAPT) is a ~76 kDa protein. Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Note For research use only.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
Images
  • Anti-Mouse Tau Recombinant Antibody (TAU-5)

    SDS-PAGE

    SDS-PAGE for Anti-Mouse MAPT/Tau/PHF-tau Antibody (TAU-5)

  • Anti-Mouse Tau Recombinant Antibody (TAU-5)

    Flow cytometry

    Flow-cytometry using anti-human Tau antibody. 5637 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Tau monoclonal antibody (Catalog MY086013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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