Anti-Human TRBC2 Antibody (SAA1764) (HP477013)

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Datasheet

Overview

Catalog No.

HP477013

Description

Anti-Human TRBC2 Antibody (SAA1764) [SAA1764] (HP477013) is a mouse monoclonal antibody detecting TRBC2 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

SAA1764

Clonality

Monoclonal

Target

TRBC2, TCRBC2, T cell receptor beta constant 2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

A0A5B9

RRID

Anti-Human TRBC2 Antibody (SAA1764) (abinScience Cat# HP477013, RRID:AB_3727525)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

T cell receptor beta constant 2 (TRBC2) is a ~19 kDa protein. Constant region of T cell receptor (TR) beta chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome.

1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447

2. Rossjohn, J. et al. (2015) Annual review of immunology 33, 169-200. PMID: 25493333

3. Brownlie, RJ. et al. (2013) Nature reviews. Immunology 13, 257-69. PMID: 23524462

4. Nikolich-Zugich, J. et al. (2004) Nature reviews. Immunology 4, 123-32. PMID: 15040585

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human TRBC2 Antibody (SAA1764).

References

Datasheet

Anti-Human TRBC2 Antibody (SAA1764).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TRBC2 Antibody (SAA1764) (HP477013)

Overview

Images

References

Datasheet

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