Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) (VK633013)

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Datasheet

Overview

Catalog No.

VK633013

Description

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) [D5] (VK633013) is a human monoclonal antibody detecting Capsid protein VP1 in ELISA, FCM, IF, WB.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human enterovirus 71 (EV71) (EV-71)

Applications

ELISA, FCM, IF, WB

Host species

Human

Isotype

IgG1

Clone ID

Clonality

Monoclonal

Target

Genome polyprotein, P1, Capsid protein VP1, P1D, Virion protein 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q5DW45

RRID

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) (abinScience Cat# VK633013, RRID:AB_3727937)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Genome polyprotein (Capsid protein VP1) is a ~242 kDa protein. Acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. During genome replication, the VPg-RNA linkage is removed by the host TDP2, thereby accelerating replication.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-EV71 VP1/Capsid protein VP1 Antibody (D5).
Bioactivity

Detects Recombinant EV71 VP1/Capsid protein VP1 Protein, N-His (Cat No.: VK633012) in indirect ELISA.

References

Datasheet

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EV71 VP1/Capsid protein VP1 Antibody (D5) (VK633013)

Overview

Images

References

Datasheet

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