Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027) (VK633033)

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Overview
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Datasheet

Overview

Catalog No.

VK633033

Description

Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027) [SAA2027] (VK633033) is a human monoclonal antibody detecting Capsid protein VP1 in ELISA, FCM, IP, Neutralization.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human enterovirus 71 (strain 7423/MS/87) (EV71) (EV-71)

Applications

ELISA, FCM, IP, Neutralization

Host species

Human

Isotype

IgG1

Clone ID

SAA2027

Clonality

Monoclonal

Target

Genome polyprotein, P1, Capsid protein VP2, P1B, Virion protein 2, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q66479

RRID

Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027) (abinScience Cat# VK633033, RRID:AB_3727939)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Genome polyprotein (Capsid protein VP 1/2) is a ~242 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1, together with VP2, interacts with host cell receptor SCARB2 to provide virion attachment to target host cells. This attachment induces virion internalization.

1. Zhou, D. et al. (2019) Nature microbiology 4, 414-419. PMID: 30531980

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027).

References

Datasheet

Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EV71 Capsid protein VP1/2/3 Antibody (SAA2027) (VK633033)

Overview

Images

References

Datasheet

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