Mouse Anti-Human CD87/PLAUR/suPAR Antibody (ATN615) (HX035013)

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Overview
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Datasheet

Overview

Catalog No.

HX035013

Description

Mouse Anti-Human CD87/PLAUR/suPAR Antibody (ATN615) [ATN615] (HX035013) is a mouse monoclonal antibody detecting MO3 in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

ATN615

Clonality

Monoclonal

Target

MO3, Urokinase plasminogen activator surface receptor, uPAR, UPAR, CD87, PLAUR, U-PAR, Monocyte activation antigen Mo3, suPAR

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q03405

RRID

Mouse Anti-Human CD87/PLAUR/suPAR Antibody (ATN615) (abinScience Cat# HX035013, RRID:AB_3727941)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Urokinase plasminogen activator surface receptor (CD87/PLAUR) is a ~36 kDa protein. Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

1. Bass, R. et al. (2005) The Journal of biological chemistry 280, 14811-8. PMID: 15677461

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Mouse Anti-Human CD87/PLAUR/suPAR Antibody (ATN615).
Bioactivity

Detects CD87 in indirect ELISA.
SDS-PAGE

SDS-PAGE for Mouse Anti-Human CD87/PLAUR/suPAR Antibody (ATN615).
Flow CytoMetry

Flow-cytometry using PE anti-human PLAUR antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human PLAUR monoclonal antibody (Catalog: HX035013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.