Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H) (JN898083)

Name: Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H)
Brand: abinScience
SKU: JN898083
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN898083

Description

Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H) (JN898083) is a mouse monoclonal antibody detecting pagA/Protective antigen in ELISA, Neutralization, WB. Suitable for Bacillus anthracis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Bacillus anthracis

Applications

ELISA, Neutralization, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a

Clone ID

83K2H

Tested applications

Target

pagA, Protective antigen, PA, Anthrax toxins translocating protein, PA-83, Bacillus anthracis

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13423

RRID

Anti-Bacillus anthracis PA/pagA/PA-83 Antibody (83K2H) (abinScience Cat# JN898083, RRID:AB_3729003)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Protective antigen (PA/pagA) is a ~85 kDa protein. Protective antigen constitutes one of the three proteins composing the anthrax toxin; it mediates attachment to host cells and translocation of edema factor (EF) and lethal factor (LF) into the host cytoplasm. PA associated with LF forms the lethal toxin (LeTx) and causes death when injected; PA associated with EF forms the edema toxin (EdTx) and produces edema. PA induces immunity to infection with anthrax.

1. Bradley, KA. et al. (2001) Nature 414, 225-9. PMID: 11700562

2. Bradley, KA. et al. (2003) The Journal of biological chemistry 278, 49342-7. PMID: 14507921

3. Santelli, E. et al. (2004) Nature 430, 905-8. PMID: 15243628

4. Lacy, DB. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 13147-51. PMID: 15326297

5. Singh, Y. et al. (1991) The Journal of biological chemistry 266, 15493-7. PMID: 1651334

6. Mock, M. et al. (2001) Annual review of microbiology 55, 647-71. PMID: 11544370

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis PA/pagA/PA-83 Recombinant Antibody (83K2H)
Bioactivity

Detects recombinant Bacillus anthracis PA/pagA/PA-83 (Catalog No: JN898022) in indirect ELISA
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Bacillus anthracis PA/pagA/PA-83(Catalog No: JN898022)
Lane 2: negative control
Lane 3: negative control

References

Datasheet

Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Bacillus anthracis pagA/Protective antigen Recombinant Antibody (83K2H) (JN898083)

Overview

Images

References

Datasheet

SDS