Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#) (JN040033)

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100ug
1mg

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Datasheet

Overview

Catalog No.

JN040033

Description

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#) [E3#] (JN040033) is a mouse monoclonal antibody detecting Bacillus anthracis in ELISA, SPR. Suitable for Bacillus anthracis.

Species reactivity

Bacillus anthracis

Applications

ELISA, SPR

Host species

Mouse

Isotype

IgG2a

Clone ID

E3#

Clonality

Monoclonal

Target

Calmodulin-sensitive adenylate cyclase, 4.6.1.1, ATP pyrophosphate-lyase, Adenylyl cyclase, Anthrax edema toxin adenylate cyclase component, Edema factor, EF, cya, pXO1-122, BXA0141, GBAA_pXO1_0142, Bacillus anthracis

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P40136

RRID

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#) (abinScience Cat# JN040033, RRID:AB_3729009)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Calmodulin-sensitive adenylate cyclase (cya/EF) is a ~92 kDa protein. Edema factor (EF), which constitutes one of the three proteins composing the anthrax toxin, causes edema in the host. Acts as a calmodulin-dependent adenylyl cyclase by converting ATP to cAMP, leading to dramatic elevation of intracellular cAMP levels in the host, thereby causing edema. EF is not toxic by itself and only acts as an edema factor when associated with protective antigen (PA) to form the edema toxin (EdTx). Required for the survival of germinated spores within macrophages at the early stages of infection.

1. Drum, CL. et al. (2002) Nature 415, 396-402. PMID: 11807546

2. Xia, ZG. et al. (1990) The Journal of biological chemistry 265, 6517-20. PMID: 2108958

3. Leppla, SH. (1982) Proceedings of the National Academy of Sciences of the United States of America 79, 3162-6. PMID: 6285339

4. Kumar, P. et al. (2001) Infection and immunity 69, 6532-6. PMID: 11553601

5. Guidi-Rontani, C. et al. (2001) Molecular microbiology 42, 931-8. PMID: 11737637

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#)

References

Datasheet

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Bacillus anthracis cya/EF/Edema factor Antibody (E3#) (JN040033)

Overview

Images

References

Datasheet

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