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Anti-Human Gal3/Galectin-3 Antibody (M3/38) (HB167023)

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Overview
Catalog No.HB167023
Species reactivityHuman
ApplicationsELISA, FCM
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDM3/38
Clonality Monoclonal
Target Mac-2 antigen, Lectin L-29, LGALS3, Galactose-specific lectin 3, 35 kDa lectin, Galactoside-binding protein, CBP 35, L-31, Gal-3, Laminin-binding protein, Galectin-3, GALBP, IgE-binding protein, Carbohydrate-binding protein 35, MAC2
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P17931
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images

  • Bioactivity

    Detects Human LGALS3 in indirect ELISAs.

  • Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Flow Cytometry

    Flow-cytometry using anti-human Galectin-3 antibody.SiHa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-3 antibody monoclonal antibody (Catalog # HB167023 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • SDS-PAGE

    SDS-PAGE for Anti-Human Gal3/Galectin-3 Antibody (M3/38).

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Anti-Human Gal3/Galectin-3 Antibody (M3/38)

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