Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154) (JN902013)

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Datasheet

Overview

Catalog No.

JN902013

Description

Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154) [SAA2154] (JN902013) is a human monoclonal antibody detecting Major surface antigen p30 in ELISA. Suitable for Toxoplasma gondii.

Species reactivity

Toxoplasma gondii

Applications

ELISA

Host species

Human

Isotype

IgG1

Clone ID

SAA2154

Clonality

Monoclonal

Target

Major surface antigen p30

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P13664

RRID

Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154) (abinScience Cat# JN902013, RRID:AB_3729523)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Major surface antigen p30 (SAG1) is a ~34 kDa protein. Mediates binding to host cells and is especially important for parasite attachment and fitness in IFN-gamma/IFNG-stimulated host cells. May regulate binding between human S100A6 and vimentin (VIM) for cytoskeleton organization during the early stages of invasion into host cells. Promotes TNF secretion in host cells probably by enhancing binding between human VIM and PRKCQ and activating NF-kappa-B pathway. Promotes viability of host cells to satisfy the survival needs of parasites through the interaction with human RACK1.

1. Robinson, SA. et al. (2004) Parasitology 128, 391-6. PMID: 15151144

2. Cohen, O. et al. (2025) Infection and immunity 93, e0001025. PMID: 40607979

3. Zhou, LJ. et al. (2021) iScience 24, 103514. PMID: 34950858

4. Wang, D. et al. (2024) Acta tropica 251, 107112. PMID: 38157925

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154)
Western blot

Western blot analysis was performed using anti-/ monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Toxoplasma gondii SAG1/Major surface antigen p30(Catalog No: JN902012)
Lane 2: negative control
Lane 3: negative control

References

Datasheet

Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Toxoplasma gondii SAG1/Major surface antigen p30 Antibody (SAA2154) (JN902013)

Overview

Images

References

Datasheet

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