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Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165) (JN041013)

Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)
Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)
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Overview
Catalog No.JN041013
Species reactivityMycobacterium tuberculosis
ApplicationsELISA, WB
Host speciesHuman
IsotypeIgG1
Clone IDSAA2165
Clonality Monoclonal
Target Catalase-peroxidase, CP, 1.11.1.21, Peroxidase/catalase, katG, Rv1908c, MTCY180.10
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P9WIE5
RRID Anti-Mycobacterium tuberculosis katG Antibody (SAA2165) (abinScience Cat# JN041013, RRID:AB_3729533)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Catalase-peroxidase (katG) is a ~80 kDa protein. Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity, oxidizing various electron donors including NADP(H). Protects M.tuberculosis against toxic reactive oxygen species (ROS) including hydrogen peroxide as well as organic peroxides and thus contributes to its survival within host macrophages by countering the phagocyte oxidative burst. Also displays efficient peroxynitritase activity, which may help the bacterium to persist in macrophages.

1. Singh, R. et al. (2008) Archives of biochemistry and biophysics 471, 207-14. PMID: 18178143
2. Johnsson, K. et al. (1997) The Journal of biological chemistry 272, 2834-40. PMID: 9006925
3. Ng, VH. et al. (2004) Molecular microbiology 52, 1291-302. PMID: 15165233
4. Sherman, DR. et al. (1996) Science (New York, N.Y.) 272, 1641-3. PMID: 8658136
5. Wengenack, NL. et al. (1999) Biochemical and biophysical research communications 256, 485-7. PMID: 10080924
Note For research use only.
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  • Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)

    SDS-PAGE

    SDS-PAGE for Anti-Mycobacterium tuberculosis katG Antibody (SAA2165)

References
Formula
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