Anti-Human CXCL4/PF4 Antibody (5B9) (HY443023)

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100ug
1mg

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Datasheet

Overview

Catalog No.

HY443023

Description

Anti-Human CXCL4/PF4 Antibody (5B9) [5B9] (HY443023) is a mouse monoclonal antibody detecting CXCL4 in ELISA. Suitable for Human.

Species reactivity

Human

Applications

ELISA

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

5B9

Clonality

Monoclonal

Target

C-X-C motif chemokine 4, PF-4, Oncostatin-A, Endothelial cell growth inhibitor, CXCL4, Iroplact, Platelet factor 4, PF4, SCYB4

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P02776

RRID

Anti-Human CXCL4/PF4 Antibody (5B9) (abinScience Cat# HY443023, RRID:AB_3729716)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Platelet factor 4 (CXCL4/PF4) is a ~10 kDa protein. Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence.

1. Fox, JM. et al. (2018) Scientific reports 8, 9466. PMID: 29930254

2. Lecomte-Raclet, L. et al. (1998) Blood 91, 2772-80. PMID: 9531587

3. Mueller, A. et al. (2008) Journal of leukocyte biology 83, 875-82. PMID: 18174362

4. Van Raemdonck, K. et al. (2014) Angiogenesis 17, 631-40. PMID: 24469069

5. Yang, C. et al. (2022) Nature communications 13, 3426. PMID: 35701499

6. Affandi, AJ. et al. (2022) Cell reports 38, 110189. PMID: 34986347

7. Dudek, AZ. et al. (1997) The Journal of biological chemistry 272, 31785-92. PMID: 9395524

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CXCL4/PF4 Antibody (5B9)

References

Datasheet

Anti-Human CXCL4/PF4 Antibody (5B9).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human CXCL4/PF4 Antibody (5B9) (HY443023)

Overview

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References

Datasheet

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