Anti-Mouse CD229/LY9 Antibody (D08) (MV366013)

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1mg

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Datasheet

Overview

Catalog No.

MV366013

Description

Anti-Mouse CD229/LY9 Antibody (D08) [D08] (MV366013) is a rabbit monoclonal antibody detecting CD229 in ELISA. Suitable for Mouse.

Highlights

●Rabbit Monoclonal — High specificity, affinity, and excellent lot-to-lot consistency.

Species reactivity

Mouse

Applications

ELISA

Host species

Rabbit

Isotype

IgG

Clone ID

D08

Clonality

Monoclonal

Target

SLAM family member 3, Lymphocyte antigen 9, Cell surface molecule Ly-9, T-lymphocyte surface antigen Ly-9, Signaling lymphocytic activation molecule 3, CD229, LY9, SLAMF3

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q01965

RRID

Anti-Mouse CD229/LY9 Antibody (D08) (abinScience Cat# MV366013, RRID:AB_3729719)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

T-lymphocyte surface antigen Ly-9 (CD229/LY9) is a ~73 kDa protein. Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A.

1. Dong, Z. et al. (2009) Nature immunology 10, 973-80. PMID: 19648922

2. de Salort, J. et al. (2013) Frontiers in immunology 4, 225. PMID: 23914190

3. Sintes, J. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 21-6. PMID: 23225888

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Mouse CD229/LY9 Antibody (D08)

References

Datasheet

Anti-Mouse CD229/LY9 Antibody (D08).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Mouse CD229/LY9 Antibody (D08) (MV366013)

Overview

Images

References

Datasheet

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