Anti-Human TSC2 IP Recombinant Antibody (SAA0237) (HW726013)

Name: Anti-Human TSC2 IP Recombinant Antibody (SAA0237)
Brand: abinScience
SKU: HW726013
Price: 191 USD
Availability: InStock

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50ug
100ug
1mg

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW726013

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Isotype

IgG1

Clone ID

SAA0237

Clonality

Monoclonal

Target

Tuberous sclerosis 2 protein, TSC2, TSC4, Tuberin

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P49815

RRID

Anti-Human TSC2 IP Antibody (SAA0237) (abinScience Cat# HW726013, RRID:AB_3729771)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Tuberin (TSC2) is a ~200 kDa protein. Catalytic component of the TSC-TBC complex, a multiprotein complex that acts as a negative regulator of the canonical mTORC1 complex, an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. Within the TSC-TBC complex, TSC2 acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In absence of nutrients, the TSC-TBC complex inhibits mTORC1, thereby preventing phosphorylation of ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) by the mTORC1 signaling. The TSC-TBC complex is inactivated in response to nutrients, relieving inhibition of mTORC1. Involved in microtubule-mediated protein transport via its ability to regulate mTORC1 signaling.

1. Inoki, K. et al. (2002) Nature cell biology 4, 648-57. PMID: 12172553

2. Tee, AR. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 13571-6. PMID: 12271141

3. Castro, AF. et al. (2003) The Journal of biological chemistry 278, 32493-6. PMID: 12842888

4. Tee, AR. et al. (2003) Current biology : CB 13, 1259-68. PMID: 12906785

5. Li, Y. et al. (2004) Molecular and cellular biology 24, 7965-75. PMID: 15340059

6. Mazhab-Jafari, MT. et al. (2012) Structure (London, England : 1993) 20, 1528-39. PMID: 22819219

7. Menon, S. et al. (2014) Cell 156, 771-85. PMID: 24529379

8. Lim, JS. et al. (2017) American journal of human genetics 100, 454-472. PMID: 28215400

9. Yang, H. et al. (2021) Nature communications 12, 339. PMID: 33436626

10. Ali, ES. et al. (2022) Molecular cell 82, 3284-3298.e7. PMID: 35772404

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human TSC2 IP Antibody (SAA0237)

References

Datasheet

Anti-Human TSC2 IP Recombinant Antibody (SAA0237).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human TSC2 IP Recombinant Antibody (SAA0237) (HW726013)

Overview

Images

References

Datasheet

SDS

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