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Anti-Sindbis virus(SINV) nsp2 Polyclonal Antibody (VK002014)

Anti-Sindbis virus(SINV) nsp2 Polyclonal Antibody
Anti-Sindbis virus(SINV) nsp2 Polyclonal Antibody
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Overview
Catalog No.VK002014
Description
Anti-Sindbis virus(SINV) nsp2 Polyclonal Antibody (VK002014) is a rabbit polyclonal antibody detecting nsp2 in WB, IHC, ELISA.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
ApplicationsWB, IHC, ELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant SINV nsp2 protein (Ala1011-Thr1353).
Target nsp2
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P03317
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polyprotein P1234 (nsp2) is a ~279 kDa protein. Inactive precursor of the viral replicase, which is activated by cleavages carried out by the viral protease nsP2.

Note For research use only.
Images
  • Anti-Sindbis virus(SINV) nsp2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NSP2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Sindbis virus(SINV) nsp2 (Catalog No: VK002012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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