Anti-Sindbis virus(SINV) Spike glycoprotein E2 Polyclonal Antibody (VK002064)

Name: Anti-Sindbis virus(SINV) Spike glycoprotein E2 Polyclonal Antibody
Brand: abinScience
SKU: VK002064
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK002064

Description

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Polyclonal Antibody (VK002064) is a rabbit polyclonal antibody detecting glycoprotein E2 in WB, IHC, ELISA.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

WB, IHC, ELISA

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant SINV glycoprotein E2 protein (Ser329-Thr695).

Target

glycoprotein E2

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Purified by antigen affinity column.

Accession

P03316

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (glycoprotein E1) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725

2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066

3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018

4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660

5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569

6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274

7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418

8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418

9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SINV-E2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Sindbis virus(SINV) Spike glycoprotein E2, N-His (Catalog No: VK002062)

References

Datasheet