Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237) (VK002063)

Name: Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237)
Brand: abinScience
SKU: VK002063
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK002063

Species reactivity

Sindbis virus (SINV)

Applications

ELISA

Host species

Mouse

Isotype

IgG, kappa

Clone ID

SAA2237

Expression system

Mammalian cells

Clonality

Monoclonal

Target

glycoprotein E2

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03316

RRID

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237) (abinScience Cat# VK002063, RRID:AB_3729847)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Structural polyprotein (glycoprotein E2) is a ~136 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Zhang, W. et al. (2002) Journal of virology 76, 11645-58. PMID: 12388725

2. Mukhopadhyay, S. et al. (2006) Structure (London, England : 1993) 14, 63-73. PMID: 16407066

3. Tang, J. et al. (2011) Journal of molecular biology 414, 442-59. PMID: 22001018

4. Paredes, AM. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 9095-9. PMID: 8415660

5. Choi, HK. et al. (1991) Nature 354, 37-43. PMID: 1944569

6. Owen, KE. et al. (1997) Virology 230, 187-96. PMID: 9143274

7. DeTulleo, L. et al. (1998) The EMBO journal 17, 4585-93. PMID: 9707418

8. Coombs, K. et al. (1987) Journal of molecular biology 195, 359-71. PMID: 3656418

9. Landers, VD. et al. (2021) Viruses 13. PMID: 33673546

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Sindbis virus(SINV) Spike glycoprotein E2 Antibody (SAA2237)

References

Datasheet

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Sindbis virus(SINV) Spike glycoprotein E2 Recombinant Antibody (SAA2237) (VK002063)

Overview

Images

References

Datasheet

SDS

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