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Anti-Vaccinia virus/VACV L4R/VP8 Polyclonal Antibody (VK005014)

Anti-Vaccinia virus/VACV L4R/VP8 Polyclonal Antibody
Anti-Vaccinia virus/VACV L4R/VP8 Polyclonal Antibody
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Overview
Catalog No.VK005014
Description
Anti-Vaccinia virus/VACV L4R/VP8 Polyclonal Antibody (VK005014) is a rabbit polyclonal antibody detecting L4R/VP8 in WB, IHC, ELISA. Suitable for Vaccinia virus and Monkeypox virus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityVaccinia virus (VACV), Monkeypox virus (MPXV)
ApplicationsWB, IHC, ELISA
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Vaccinia virus/VACV L4R/VP8 (Ala33-Asp251).
Target L4R/VP8
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession P03295
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCore protein VP8, 25 kDa major core protein, F5 polypeptide, L4 core protein, P25K, OPG098
Background

Core protein VP8 (L4R) is a ~28 kDa protein. Major core structural protein.

Note For research use only.
Images
  • Anti-Vaccinia virus/VACV L4R/VP8 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-VP8-VACCW polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant Vaccinia virus/VACV L4R/VP8 (Catalog No: VK005012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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