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Anti-Zebrafish mapk1 Polyclonal Antibody (ZA456014)

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Overview
Catalog No.ZA456014
Description
Anti-Zebrafish mapk1 Polyclonal Antibody (ZA456014) is a rabbit polyclonal antibody detecting ERK2; MAPK1; p42MAPK in ELISA, IHC, WB. Suitable for Danio rerio, Mus musculus, Bovine, Xenopus laevis, and Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityZebrafish
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Zebrafish mapk1 (Met117-His319).
Target extracellular signal-regulated kinase 2 [Danio rerio], identical sequence found in cell line ZF4; primers designed on EST AB030903, Catalytic domain of Extracellular signal-Regulated Kinase 1 and 2-like Serine/Threonine Kinases; cd07849, ATP binding site [chemical binding], polypeptide substrate binding site [polypeptide binding], KIM docking site [polypeptide binding], activation loop (A-loop), ERK2; MAPK1; p42MAPK
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession AAY57805.1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Namesextracellular signal-regulated kinase 2, extracellular signal-regulated kinase 2
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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