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Anti-Zebrafish wnt3a Polyclonal Antibody (ZA461014)

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Overview
Catalog No.ZA461014
Description
Anti-Zebrafish wnt3a Polyclonal Antibody (ZA461014) is a rabbit polyclonal antibody detecting wnt3a in ELISA, IHC, WB. Suitable for Danio rerio, Meleagris gallopavo, Plethodon jordani, Alopias vulpinus, and Gallus gallus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityDanio rerio (Zebrafish)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Species Danio rerio (Zebrafish)
Clonality Polyclonal
Immunogen E. coli - derived recombinant Zebrafish wnt3a (Val50-Ala250).
Target protein Wnt-3a precursor [Danio rerio], wnt3a, wingless-type MMTV integration site family, member 3 like; wnt3 like, Wnt domain found in proto-oncogene Wnt-3 and similar proteins; cd19335, Frizzled receptor binding site [polypeptide binding]
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession NP_001007186.1
RRID Anti-Zebrafish wnt3a Polyclonal Antibody (abinScience Cat# ZA461014, RRID:AB_3729922)
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Namesprotein Wnt-3a precursor, wnt3a
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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