Anti-Human EPRS1 Polyclonal Antibody (HY479014)

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Datasheet

Overview

Catalog No.

HY479014

Description

Anti-Human EPRS1 Polyclonal Antibody (HY479014) is a rabbit polyclonal antibody detecting EPRS1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Homo sapiens

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human EPRS1 (Asp749-Thr956).

Target

EPRS1

Purification

Purified by antigen affinity column.

Accession

P07814

RRID

Anti-Human EPRS1 Polyclonal Antibody (abinScience Cat# HY479014, RRID:AB_3729953)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -83°C for twelve months from the date of receipt.

Alternate Names

Bifunctional glutamate/proline--tRNA ligase, Bifunctional aminoacyl-tRNA synthetase, Cell proliferation-inducing gene 32 protein, Glutamatyl-prolyl-tRNA synthetase, Glutamate--tRNA ligase, 6.1.1.17, Glutamyl-tRNA synthetase, GluRS, Proline--tRNA ligase, 6.1.1.15, Prolyl-tRNA synthetase, EPRS1, EPRS, GLNS, PARS, QARS, QPRS, PIG32

Background

Bifunctional glutamate/proline--tRNA ligase (EPRS1) is a ~170 kDa protein. Multifunctional protein which primarily functions within the aminoacyl-tRNA synthetase multienzyme complex, also known as multisynthetase complex. Within the complex it catalyzes the attachment of both L-glutamate and L-proline to their cognate tRNAs in a two-step reaction where the amino acid is first activated by ATP to form a covalent intermediate with AMP. Subsequently, the activated amino acid is transferred to the acceptor end of the cognate tRNA to form L-glutamyl-tRNA(Glu) and L-prolyl-tRNA(Pro). Upon interferon-gamma stimulation, EPRS1 undergoes phosphorylation, causing its dissociation from the aminoacyl-tRNA synthetase multienzyme complex. It is recruited to form the GAIT complex, which binds to stem loop-containing GAIT elements found in the 3'-UTR of various inflammatory mRNAs, such as ceruloplasmin.

1. Zhou, H. et al. (2013) Nature 494, 121-4. PMID: 23263184

2. Son, J. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2136-45. PMID: 24100331

3. Mendes, MI. et al. (2018) American journal of human genetics 102, 676-684. PMID: 29576217

4. Thömmes, P. et al. (1988) Nucleic acids research 16, 5391-406. PMID: 3290852

5. Yoon, I. et al. (2023) EMBO molecular medicine 15, e16940. PMID: 37212275

6. Sampath, P. et al. (2004) Cell 119, 195-208. PMID: 15479637

7. Arif, A. et al. (2012) Molecular and cellular biology 32, 5046-55. PMID: 23071094

8. Arif, A. et al. (2017) Nature 542, 357-361. PMID: 28178239

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human EPRS1 polyclonal antibody at 1ug/mL on recombinant Human EPRS1 (Catalog No. HY479012).

References

Datasheet

Anti-Human EPRS1 Polyclonal Antibody.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human EPRS1 Polyclonal Antibody (HY479014)

Overview

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References

Datasheet

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