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Anti-Human PLCB1 Polyclonal Antibody (HV228014)

Anti-Human PLCB1 Polyclonal Antibody
Anti-Human PLCB1 Polyclonal Antibody
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Overview
Catalog No.HV228014
Description
Anti-Human PLCB1 Polyclonal Antibody (HV228014) is a rabbit polyclonal antibody detecting PLCB1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Bovine, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PLCB1 (Lys27-Met245).
Target PLCB1
Purification Purified by antigen affinity column.
Accession Q9NQ66
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase beta-1, 3.1.4.11, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1, PLCB1, KIAA0581
Background

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1 (PLCB1) is a ~138 kDa protein. Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors. Regulates the function of the endothelial barrier.

1. Tall, E. et al. (1997) Biochemistry 36, 7239-48. PMID: 9188725
Note For research use only.
Images
  • Anti-Human PLCB1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PLCB1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate
    Lane 3: Rat cerebellum lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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